Material Safety Data Sheet
EDTA 0.5M Solution of pH 8.0 (for DNA Work)

ACC# 89854
Section 1 - Chemical Product and Company Identification

MSDS Name: EDTA 0.5M Solution of pH 8.0 (for DNA Work)
Catalog Numbers: BP2482-1, BP2482-100, BP2482-20, BP2482-4, BP2482-500

Company Identification:

              Fisher Scientific
              1 Reagent Lane
              Fair Lawn, NJ 07410
For information, call:
Emergency Number:
For CHEMTREC assistance, call:
For International CHEMTREC assistance, call:

Section 2 - Composition, Information on Ingredients

CAS# Chemical Name Percent EINECS/ELINCS
7732-18-5 Water >98 231-791-2
60-00-4 Ethylenediaminetetraacetic acid (EDTA) <2.0 200-449-4

Section 3 - Hazards Identification


Appearance: Not available.
Warning! May cause allergic skin reaction. May cause eye, skin, and respiratory tract irritation. May cause kidney damage. May cause reproductive and fetal effects.
Target Organs: Kidneys, bone marrow.

Potential Health Effects
Eye: May cause eye irritation.
Skin: May cause skin irritation. May cause skin sensitization, an allergic reaction, which becomes evident upon re-exposure to this material.
Ingestion: May cause irritation of the digestive tract. Exposure may cause kidney injury, muscle cramps, bone-marrow depression, and a generalized allergic
Inhalation: May cause respiratory tract irritation.
Chronic: Prolonged or repeated skin contact may cause sensitization dermatitis and possible destruction and/or ulceration.

Section 4 - First Aid Measures

Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid imme diately.
Skin: Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Get medical aid if irritation develops or persists.
Ingestion: If victim is conscious and alert, give 2-4 cupfuls of milk or water. Never give anything by mouth to an unconscious person. Get medical aid immediately.
Inhalation: Remove from exposure and move to fresh air immediately. Get medical aid if cough or other symptoms appear.
Notes to Physician: Treat symptomatically and supportively.

Section 5 - Fire Fighting Measures

General Information: As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. Non-combustible, substance itself does not burn but may decompose upon heating to produce irritating, corrosive and/or toxic fumes.
Extinguishing Media: Substance is noncombustible; use agent most appropriate to extinguish surrounding fire. Use water spray, dry chemical, carbon dioxide, or chemical foam.
Flash Point: Not applicable.
Autoignition Temperature: Not applicable.
Explosion Limits, Lower:Not available.
Upper: Not available.
NFPA Rating: (estimated) Health: 1; Flammability: 0; Instability: 0

Section 6 - Accidental Release Measures

General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks: Sweep up or absorb material, then place into a suitable clean, dry, closed container for disposal.

Section 7 - Handling and Storage

Handling: Wash thoroughly after handling. Use with adequate ventilation. Avoid contact with skin and eyes. Keep container tightly closed. Avoid ingestion and inhalation.
Storage: Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances.

Section 8 - Exposure Controls, Personal Protection

Engineering Controls: Use adequate ventilation to keep airborne concentrations low.
Exposure Limits
Chemical Name ACGIH NIOSH OSHA - Final PELs
Water none listed none listed none listed
Ethylenediaminetetraacetic acid (EDTA) none listed none listed none listed

OSHA Vacated PELs: Water: No OSHA Vacated PELs are listed for this chemical. Ethylenediaminetetraacetic acid (EDTA): No OSHA Vacated PELs are listed for this chemical.
Personal Protective Equipment
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin: Wear appropriate protective gloves to prevent skin exposure.
Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators: Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced.

Section 9 - Physical and Chemical Properties

Physical State: Liquid
Appearance: Not available.
Odor: Not available.
pH: 8
Vapor Pressure: Not available.
Vapor Density: Not available.
Evaporation Rate:Not available.
Viscosity: Not available.
Boiling Point: Not available.
Freezing/Melting Point:Not available.
Decomposition Temperature:Not available.
Solubility: Not available.
Specific Gravity/Density:Not available.
Molecular Formula:Solution
Molecular Weight:Not available.

Section 10 - Stability and Reactivity

Chemical Stability: Stable under normal temperatures and pressures.
Conditions to Avoid: Incompatible materials.
Incompatibilities with Other Materials: Strong oxidizing agents, strong bases, copper, copper alloys, aluminum.
Hazardous Decomposition Products: Nitrogen oxides, carbon monoxide, carbon dioxide.
Hazardous Polymerization: Has not been reported.

Section 11 - Toxicological Information

CAS# 7732-18-5: ZC0110000      
CAS# 60-00-4: AH4025000
CAS# 7732-18-5:
     Oral, rat: LD50 = >90 mL/kg;

CAS# 60-00-4:
     Oral, mouse: LD50 = 30 mg/kg;

CAS# 7732-18-5: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 60-00-4: Not listed by ACGIH, IARC, NTP, or CA Prop 65.

Epidemiology: No information available.
Teratogenicity: Embryo or Fetus: Stunted fetus, oral-rat TDLo=7632mg/kg. Specific Developmental Abnormalities: Cardiovascular, Craniofacial, Musculoskeletal, Respiratory, and Urogenital, oral-rat TDLo=7632mg/kg.
Reproductive Effects: Fertility: Post-implantation mortality, oral-rat TDLo=7632mg/kg.
Mutagenicity: Cytogenetic Analysis: intraperitoneal-mouse 50mmol/L. DNA Inhibition: hamster fibroblast 500ug/L, rabbit kidney 250umol/L.
Neurotoxicity: No information available.
Other Studies:

Section 12 - Ecological Information

Ecotoxicity: No data available. Catfish (tap water) 129ppm/96H.
Environmental: Biological Oxygen Demand (BOD): 1%, 5 days.
Physical: No information available.
Other: None.

Section 13 - Disposal Considerations

Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.
RCRA P-Series: None listed.
RCRA U-Series: None listed.

Section 14 - Transport Information

Shipping Name: Not regulated as a hazardous material No information available.
Hazard Class:
UN Number:
Packing Group:

Section 15 - Regulatory Information


     CAS# 7732-18-5 is listed on the TSCA inventory.
     CAS# 60-00-4 is listed on the TSCA inventory.
Health & Safety Reporting List
     None of the chemicals are on the Health & Safety Reporting List.
Chemical Test Rules
     None of the chemicals in this product are under a Chemical Test Rule.
Section 12b
     None of the chemicals are listed under TSCA Section 12b.
TSCA Significant New Use Rule
     None of the chemicals in this material have a SNUR under TSCA.
CERCLA Hazardous Substances and corresponding RQs
     CAS# 60-00-4: 5000 lb final RQ; 2270 kg final RQ
SARA Section 302 Extremely Hazardous Substances
     None of the chemicals in this product have a TPQ.
Section 313      No chemicals are reportable under Section 313.
Clean Air Act:
     This material does not contain any hazardous air pollutants.
     This material does not contain any Class 1 Ozone depletors.
     This material does not contain any Class 2 Ozone depletors.
Clean Water Act:
CAS# 60-00-4 is listed as a Hazardous Substance under the CWA.
     None of the chemicals in this product are listed as Priority Pollutants under the CWA.
     None of the chemicals in this product are listed as Toxic Pollutants under the CWA.
     None of the chemicals in this product are considered highly hazardous by OSHA.
     CAS# 7732-18-5 is not present on state lists from CA, PA, MN, MA, FL, or NJ.
     CAS# 60-00-4 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Massachusetts.

California Prop 65

California No Significant Risk Level: None of the chemicals in this product are listed.

European/International Regulations

European Labeling in Accordance with EC Directives
Hazard Symbols:
      Not available.
Risk Phrases:

Safety Phrases:

     S 24/25 Avoid contact with skin and eyes.

WGK (Water Danger/Protection)

     CAS# 7732-18-5: No information available.
     CAS# 60-00-4: 2
Canada - DSL/NDSL
     CAS# 7732-18-5 is listed on Canada's DSL List.
     CAS# 60-00-4 is listed on Canada's DSL List.
Canada - WHMIS
     not available.
This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations and the MSDS contains all of the information required by those regulations.
Canadian Ingredient Disclosure List

Section 16 - Additional Information

MSDS Creation Date: 2/16/1998
Revision #7 Date: 10/30/2007

The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. In no event shall Fisher be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages, howsoever arising, even if Fisher has been advised of the possibility of such damages.