Material Safety Data Sheet
Phenolphthalein Solution 1%

ACC# 45376
Section 1 - Chemical Product and Company Identification

MSDS Name: Phenolphthalein Solution 1%
Catalog Numbers: SP62-1, SP62-500
Phenolphthalein Indicator Solution
Company Identification:

              Fisher Scientific
              1 Reagent Lane
              Fair Lawn, NJ 07410
For information, call:
Emergency Number:
For CHEMTREC assistance, call:
For International CHEMTREC assistance, call:

Section 2 - Composition, Information on Ingredients

CAS# Chemical Name Percent EINECS/ELINCS
67-63-0 Isopropyl alcohol 99 200-661-7
77-09-8 Phenolphthalein 1 201-004-7

Section 3 - Hazards Identification


Appearance: colorless liquid. Flash Point: 12 deg C.
Warning! Flammable liquid and vapor. Causes eye, skin, and respiratory tract irritation. May cause allergic skin reaction. May cause central nervous system depression. May cause cancer based on animal studies. May form explosive peroxides. May cause kidney damage. May cause reproductive and fetal effects.
Target Organs: Kidneys, central nervous system.

Potential Health Effects
Eye: Produces irritation, characterized by a burning sensation, redness, tearing, inflammation, and possible corneal injury.
Skin: May cause skin sensitization, an allergic reaction, which becomes evident upon re-exposure to this material. Prolonged and/or repeated contact may cause defatting of the skin and dermatitis. May cause irritation with pain and stinging, especially if the skin is abraded.
Ingestion: May cause gastrointestinal irritation with nausea, vomiting and diarrhea. May cause kidney damage. May cause central nervous system depression, characterized by excitement, followed by headache, dizziness, drowsiness, and nausea. Advanced stages may cause collapse, unconsciousness, coma and possible death due to respiratory failure.
Inhalation: Inhalation of high concentrations may cause central nervous system effects characterized by nausea, headache, dizziness, unconsciousness and coma. Inhalation of vapor may cause respiratory tract irritation.
Chronic: Possible cancer hazard based on tests with laboratory animals. Prolonged or repeated skin contact may cause defatting and dermatitis. May cause kidney injury. May cause allergic skin reaction in some individuals.

Section 4 - First Aid Measures

Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid.
Skin: Flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Get medical aid if irritation develops or persists.
Ingestion: Do not induce vomiting. If victim is conscious and alert, give 2-4 cupfuls of milk or water. Never give anything by mouth to an unconscious person. Get medical aid immediately.
Inhalation: Get medical aid immediately. Remove from exposure and move to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Do NOT use mouth-to-mouth resuscitation.
Notes to Physician: Treat symptomatically and supportively.

Section 5 - Fire Fighting Measures

General Information: As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. Vapors can travel to a source of ignition and flash back. Use water spray to keep fire-exposed containers cool. Containers may explode in the heat of a fire. This chemical poses an explosion hazard. May form explosive peroxides. Vapors may be heavier than air. They can spread along the ground and collect in low or confined areas.
Extinguishing Media: For small fires, use dry chemical, carbon dioxide, water spray or alcohol-resistant foam. Water may be ineffective. For large fires, use water spray, fog or alcohol-resistant foam. Do NOT use straight streams of water. Cool containers with flooding quantities of water until well after fire is out.
Flash Point: 12 deg C ( 53.60 deg F)
Autoignition Temperature: 398.9 deg C ( 750.02 deg F)
Explosion Limits, Lower:2.0
Upper: 12.7
NFPA Rating: (estimated) Health: 1; Flammability: 3; Instability: 0

Section 6 - Accidental Release Measures

General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks: Absorb spill with inert material (e.g. vermiculite, sand or earth), then place in suitable container. Avoid runoff into storm sewers and ditches which lead to waterways. Clean up spills immediately, observing precautions in the Protective Equipment section. Scoop up with a nonsparking tool, then place into a suitable container for disposal. Remove all sources of ignition. Provide ventilation.

Section 7 - Handling and Storage

Handling: Wash thoroughly after handling. Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Use with adequate ventilation. Use spark-proof tools and explosion proof equipment. Avoid contact with eyes, skin, and clothing. Empty containers retain product residue, (liquid and/or vapor), and can be dangerous. Keep container tightly closed. Avoid ingestion and inhalation. Do not pressurize, cut, weld, braze, solder, drill, grind, or expose empty containers to heat, sparks or open flames.
Storage: Keep away from heat, sparks, and flame. Keep away from sources of ignition. Keep container closed when not in use. Keep from contact with oxidizing materials. Store in a cool, dry, well-ventilated area away from incompatible substances. Flammables-area.

Section 8 - Exposure Controls, Personal Protection

Engineering Controls: Good general ventilation should be sufficient to control airborne levels. Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower.
Exposure Limits
Chemical Name ACGIH NIOSH OSHA - Final PELs
Isopropyl alcohol 200 ppm TWA; 400 ppm STEL 400 ppm TWA; 980 mg/m3 TWA 2000 ppm IDLH (10% LEL) 400 ppm TWA; 980 mg/m3 TWA
Phenolphthalein none listed none listed none listed

OSHA Vacated PELs: Isopropyl alcohol: 400 ppm TWA; 980 mg/m3 TWA Phenolphthalein: No OSHA Vacated PELs are listed for this chemical.
Personal Protective Equipment
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin: Nitrile or Neoprene gloves are recommended.
Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators: Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced.

Section 9 - Physical and Chemical Properties

Physical State: Liquid
Appearance: colorless
Odor: alcohol-like
pH: Not available.
Vapor Pressure: 40 mm Hg
Vapor Density: 2.1
Evaporation Rate:2.88 (Butyl Acetate=1)
Viscosity: Not available.
Boiling Point: 83 deg C
Freezing/Melting Point:-89 deg C
Decomposition Temperature:Not available.
Solubility: Soluble in water.
Specific Gravity/Density:0.7855
Molecular Formula:Mixture
Molecular Weight:Not available.

Section 10 - Stability and Reactivity

Chemical Stability: Stable. This material may be sensitive to peroxide formation.
Conditions to Avoid: This material may be sensitive to peroxide formation., incompatible materials, ignition sources, excess heat.
Incompatibilities with Other Materials: Oxidizing agents, Isopropanol is susceptible to autoxidation and therefore should be classified as peroxidizable..
Hazardous Decomposition Products: Carbon monoxide, carbon dioxide, acrid smoke and fumes.
Hazardous Polymerization: Will not occur.

Section 11 - Toxicological Information

CAS# 67-63-0: NT8050000      
CAS# 77-09-8: SM8380000
CAS# 67-63-0:
     Draize test, rabbit, eye: 100 mg Severe;
     Draize test, rabbit, eye: 10 mg Moderate;
     Draize test, rabbit, eye: 100 mg/24H Moderate;
     Draize test, rabbit, skin: 500 mg Mild;
     Inhalation, mouse: LC50 = 53000 mg/m3;
     Inhalation, rat: LC50 = 16000 ppm/8H;
     Inhalation, rat: LC50 = 72600 mg/m3;
     Oral, mouse: LD50 = 3600 mg/kg;
     Oral, mouse: LD50 = 3600 mg/kg;
     Oral, rabbit: LD50 = 6410 mg/kg;
     Oral, rat: LD50 = 5045 mg/kg;
     Oral, rat: LD50 = 5000 mg/kg;
     Skin, rabbit: LD50 = 12800
CAS# 77-09-8:

CAS# 67-63-0: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 77-09-8:

Epidemiology: The NTP reported that there was clear evidence of carcinogenic activity in male rats based on the markedly increased incidences of benign pheochromocytoma of the adrenal medulla and others. There was clear evidence in mice based on the increased incidences of histiocytic sarcoma and malignant lymphoma of thymic origin. There was also clear evidence in female mice based on the increased incidences of histiocytic sarcoma, malignant lymphomas, and benign sex-cord stromal tumors of the ovary.
Teratogenicity: No information available.
Reproductive Effects: No information available.
Mutagenicity: Significant increases in chromosomal aberrations were observed after treament of cultured Chinese hamster ovary cells with phenolphthalein in the presence of S9. Frequencies of micronucleated erythrocytes were noted in male and female feeding studies.
Neurotoxicity: No information available.
Other Studies:

Section 12 - Ecological Information

Ecotoxicity: No data available. Cas# 67-63-0:LC50 (96Hr.) Fathead Minnow = 94900-10400 mg/L; Flow-through conditionLC50 (96 Hr.) Fathead Minnow = 61200-65500 mg/L;Flow-through condition.
Environmental: Cas# 67-63-0: TERRESTRIAL FATE: When spilled on soil, isopropanol will both evaporate quickly and leach into the ground due to its high vapor pressure and low adsorption to soil. Degradation in soil and groundwater has not been determined. If soil degradation is not rapid, it is apt to leach into the groundwater. AQUATIC FATE: When released into water, isopropyl alcohol will volatilize (estimated half-life approximately 5.4 days) and may biodegrade. Although it is readily degradable in a number of laboratory tests, no data on its degradability in natural waters.
Physical: Cas# 67-63-0: ATMOSPHERIC FATE: When released into the atmosphere, isopropanol will photodegrade with an estimated half-life ranging from one to several days. Due to its solubility in water, rainout may be significant.
Other: No information available.

Section 13 - Disposal Considerations

Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.
RCRA P-Series: None listed.
RCRA U-Series: None listed.

Section 14 - Transport Information

Hazard Class: 3 3
UN Number: UN1219 UN1219
Packing Group: II II

Section 15 - Regulatory Information


     CAS# 67-63-0 is listed on the TSCA inventory.
     CAS# 77-09-8 is listed on the TSCA inventory.
Health & Safety Reporting List
     CAS# 67-63-0: Effective 12/15/86, Sunset 12/15/96
Chemical Test Rules
     CAS# 67-63-0: 40 CFR 799.2325
Section 12b
     None of the chemicals are listed under TSCA Section 12b.
TSCA Significant New Use Rule
     None of the chemicals in this material have a SNUR under TSCA.
CERCLA Hazardous Substances and corresponding RQs
     None of the chemicals in this material have an RQ.
SARA Section 302 Extremely Hazardous Substances
     None of the chemicals in this product have a TPQ.
SARA Codes
     CAS # 67-63-0: immediate, delayed, fire.
     CAS # 77-09-8: immediate.
Section 313
     This material contains Isopropyl alcohol (CAS# 67-63-0, 99%),which is subject to the reporting requirements of Section 313 of SARA Title III and 40 CFR Part 373.
Clean Air Act:
     This material does not contain any hazardous air pollutants.
     This material does not contain any Class 1 Ozone depletors.
     This material does not contain any Class 2 Ozone depletors.
Clean Water Act:
     None of the chemicals in this product are listed as Hazardous Substances under the CWA.
     None of the chemicals in this product are listed as Priority Pollutants under the CWA.
     None of the chemicals in this product are listed as Toxic Pollutants under the CWA.
     None of the chemicals in this product are considered highly hazardous by OSHA.
     CAS# 67-63-0 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts.
     CAS# 77-09-8 is not present on state lists from CA, PA, MN, MA, FL, or NJ.

California Prop 65
WARNING: This product contains Phenolphthalein, a chemical known to the state of California to cause cancer.
California No Significant Risk Level: None of the chemicals in this product are listed.

European/International Regulations

European Labeling in Accordance with EC Directives
Hazard Symbols:
Risk Phrases:

     R 11 Highly flammable.

Safety Phrases:

     S 16 Keep away from sources of ignition - No smoking.
     S 24/25 Avoid contact with skin and eyes.

WGK (Water Danger/Protection)

     CAS# 67-63-0: 1
     CAS# 77-09-8: 1
Canada - DSL/NDSL
     CAS# 67-63-0 is listed on Canada's DSL List.
     CAS# 77-09-8 is listed on Canada's DSL List.
Canada - WHMIS
     This product has a WHMIS classification of B2, D2B.
This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations and the MSDS contains all of the information required by those regulations.
Canadian Ingredient Disclosure List
     CAS# 67-63-0 is listed on the Canadian Ingredient Disclosure List.

Section 16 - Additional Information

MSDS Creation Date: 7/27/1999
Revision #7 Date: 2/15/2008

The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. In no event shall Fisher be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages, howsoever arising, even if Fisher has been advised of the possibility of such damages.