Material Safety Data Sheet
TBE Buffer Mix

ACC# 61029
Section 1 - Chemical Product and Company Identification

MSDS Name: TBE Buffer Mix
Catalog Numbers: BP1396-86, BP1396ME
Dry Powder Mix of Tris/ Basic Acid EDTA
Company Identification:

              Fisher Scientific
              1 Reagent Lane
              Fair Lawn, NJ 07410
For information, call:
Emergency Number:
For CHEMTREC assistance, call:
For International CHEMTREC assistance, call:

Section 2 - Composition, Information on Ingredients

CAS# Chemical Name Percent EINECS/ELINCS
77-86-1 Tris (hydroxymethyl) aminomethane 63.91 201-064-4
10043-35-3 Boric acid 32.54 233-139-2
6381-92-6 Disodium ethylenediaminetetraacetate dihydrate 3.55 unlisted

Section 3 - Hazards Identification


Appearance: white solid.
Warning! Causes eye, skin, and respiratory tract irritation. May impair fertility. May cause harm to the unborn child. May cause central nervous system effects.
Target Organs: Central nervous system, respiratory system, eyes, reproductive system, skin.

Potential Health Effects
Eye: Causes eye irritation.
Skin: Causes skin irritation. May be absorbed through damaged or abraded skin in harmful amounts.
Ingestion: May cause gastric disturbances and electrolytic imbalance. May cause cyanosis (bluish discoloration of skin due to deficient oxygenation of the blood). Boric acid poisoning begins with nausea, vomiting and diarrhea. There is a red skin rash followed by extensive exfoliation not only in areas of rash but also of mucous membranes. Other symptoms may include weakness, headache, restlessness & kidney injury CNS effects (excitement or depression, lethargy, headache, coma, seizures), dehydration, arrhythmias, shock and metabolic acidosis have been reported in extreme adult and pediatric cases.
Inhalation: Causes respiratory tract irritation.
Chronic: Prolonged or repeated skin contact may cause dermatitis. Chronic poisoning by boron compounds, borism, may be little more than dry skin and mucous membranes, followed by appearance of a red tongue, patchy alopecia (hair loss), cracked lips, and conjunctivitis. Infants and young children are more susceptible to boric acid poisoning than adults. May cause adverse reproductive effects.

Section 4 - First Aid Measures

Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid.
Skin: Get medical aid. Flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse.
Ingestion: Never give anything by mouth to an unconscious person. Get medical aid. Do NOT induce vomiting. If conscious and alert, rinse mouth and drink 2-4 cupfuls of milk or water.
Inhalation: Remove from exposure and move to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid.
Notes to Physician: Treat symptomatically and supportively.

Section 5 - Fire Fighting Measures

General Information: As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion.
Extinguishing Media: Use water spray, dry chemical, carbon dioxide, or chemical foam.
Flash Point: Not applicable.
Autoignition Temperature: Not applicable.
Explosion Limits, Lower:Not available.
Upper: Not available.
NFPA Rating: (estimated) Health: 2; Flammability: 0; Instability: 1

Section 6 - Accidental Release Measures

General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks: Vacuum or sweep up material and place into a suitable disposal container. Clean up spills immediately, observing precautions in the Protective Equipment section. Avoid generating dusty conditions. Provide ventilation.

Section 7 - Handling and Storage

Handling: Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Use with adequate ventilation. Minimize dust generation and accumulation. Avoid breathing dust, mist, or vapor. Avoid contact with eyes, skin, and clothing. Keep container tightly closed. Discard contaminated shoes.
Storage: Store in a cool, dry, well-ventilated area away from incompatible substances. Keep containers tightly closed.

Section 8 - Exposure Controls, Personal Protection

Engineering Controls: Use process enclosure, local exhaust ventilation, or other engineering controls to control airborne levels below recommended exposure limits. Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower.
Exposure Limits
Chemical Name ACGIH NIOSH OSHA - Final PELs
Tris (hydroxymethyl) aminomethane none listed none listed none listed
Boric acid 2 mg/m3 TWA (inhalable fraction, listed under Borate compounds, inorganic); 6 mg/m3 STEL (inhalable fraction, listed under Borate compounds, inorganic) none listed none listed
Disodium ethylenediaminetetraacetate dihydrate none listed none listed none listed

OSHA Vacated PELs: Tris (hydroxymethyl) aminomethane: No OSHA Vacated PELs are listed for this chemical. Boric acid: No OSHA Vacated PELs are listed for this chemical. Disodium ethylenediaminetetraacetate dihydrate: No OSHA Vacated PELs are listed for this chemical.
Personal Protective Equipment
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin: Wear appropriate protective gloves to prevent skin exposure.
Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators: Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced.

Section 9 - Physical and Chemical Properties

Physical State: Solid
Appearance: white
Odor: odorless
pH: Not available.
Vapor Pressure: Not available.
Vapor Density: Not available.
Evaporation Rate:Not applicable.
Viscosity: Not available.
Boiling Point: Not applicable.
Freezing/Melting Point:Not available.
Decomposition Temperature:Not available.
Solubility: Soluble in water.
Specific Gravity/Density:Not available.
Molecular Formula:Mixture
Molecular Weight:Not available

Section 10 - Stability and Reactivity

Chemical Stability: Stable at room temperature in closed containers under normal storage and handling conditions. Decarboxylates above 150C. Boric acid is a stable product, but when heated, it loses water to form metaboric acid (HBO2), and on further heating it is converted into boric oxide (B2O3).
Conditions to Avoid: High temperatures, incompatible materials, dust generation, exposure to moist air or water.
Incompatibilities with Other Materials: Reaction with strong reducing agents, such as metal hydrides or alkali metals, will generate hydrogen gas, which could create an explosion hazard., caustics (e.g. ammonia, ammonium hydroxide, calcium hydroxide, potassium hydroxide, sodium hydroxide), acetic anhydride, alkali carbonates.
Hazardous Decomposition Products: Carbon monoxide, carbon dioxide, nitrogen oxides (NOx) and ammonia (NH3), oxides of boron.
Hazardous Polymerization: Has not been reported.

Section 11 - Toxicological Information

CAS# 77-86-1: TY2900000      
CAS# 10043-35-3: ED4550000; ED4560000      
CAS# 6381-92-6: AH4410000
CAS# 77-86-1:
     Oral, rat: LD50 = 5900 mg/kg;

CAS# 10043-35-3:
     Oral, mouse: LD50 = 3450 mg/kg;
     Oral, rat: LD50 = 2660 mg/kg;
     Oral, rat: LD50 = 2500 mg/kg;

CAS# 6381-92-6:

CAS# 77-86-1: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 10043-35-3: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 6381-92-6: Not listed by ACGIH, IARC, NTP, or CA Prop 65.

Epidemiology: Weakened sexual activity and a low level of genital functions (low sperm counts and motility) were observed in 28 male workers engaged in the production of boric acid. Due to a lack of detailed description of this observation, its value is limited.
Teratogenicity: Developmental effects were observed in mice, rats and rabbits after oral adminstration of boric acid. However, these effects were considered secondary to maternal toxicity (increased liver and kidney weight).
Reproductive Effects: Boric acid was found to induce testicular atrophy and effects on spermatogenesis in rats and mice in various studies. Effects occurred at dose-levels (27 mg/kg) without general toxicity.Boric acid has selectively damaged the testes, sperm production and fertility in rats and dogs.
Mutagenicity: No information found
Neurotoxicity: No information found
Other Studies:

Section 12 - Ecological Information

Ecotoxicity: Water flea Daphnia: LC50 = 115.0-153.0 mg/L; 48 Hr.; Static ConditionFish: Rainbow trout: LC50=150mg B/L; 24-day; Fish: Goldfish: LC50=46mg B/L; 7-day; Mosquito fish (fresh water) TLm=1800 ppm/24H Mosquito fish (fresh water) TLm=1800 ppm/24H
Environmental: Boric acid is a water-soluble white powder that may, at high concentrations, cause damage to trees or vegetation by root absorption.
Physical: No information available.
Other: None.

Section 13 - Disposal Considerations

Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.
RCRA P-Series: None listed.
RCRA U-Series: None listed.

Section 14 - Transport Information

Shipping Name: Not Regulated Not Regulated
Hazard Class:
UN Number:
Packing Group:

Section 15 - Regulatory Information


     CAS# 77-86-1 is listed on the TSCA inventory.
     CAS# 10043-35-3 is listed on the TSCA inventory.
     CAS# 6381-92-6 is not on the TSCA Inventory because it is a hydrate. It is considered to be listed if the CAS number for the anhydrous form is on the inventory (40CFR720.3(u)(2)).
Health & Safety Reporting List
     None of the chemicals are on the Health & Safety Reporting List.
Chemical Test Rules
     None of the chemicals in this product are under a Chemical Test Rule.
Section 12b
     None of the chemicals are listed under TSCA Section 12b.
TSCA Significant New Use Rule
     None of the chemicals in this material have a SNUR under TSCA.
CERCLA Hazardous Substances and corresponding RQs
     None of the chemicals in this material have an RQ.
SARA Section 302 Extremely Hazardous Substances
     None of the chemicals in this product have a TPQ.
SARA Codes
     CAS # 77-86-1: immediate.
     CAS # 10043-35-3: immediate, delayed.
     CAS # 6381-92-6: immediate.
Section 313      No chemicals are reportable under Section 313.
Clean Air Act:
     This material does not contain any hazardous air pollutants.
     This material does not contain any Class 1 Ozone depletors.
     This material does not contain any Class 2 Ozone depletors.
Clean Water Act:
     None of the chemicals in this product are listed as Hazardous Substances under the CWA.
     None of the chemicals in this product are listed as Priority Pollutants under the CWA.
     None of the chemicals in this product are listed as Toxic Pollutants under the CWA.
     None of the chemicals in this product are considered highly hazardous by OSHA.
     CAS# 77-86-1 is not present on state lists from CA, PA, MN, MA, FL, or NJ.
     CAS# 10043-35-3 is not present on state lists from CA, PA, MN, MA, FL, or NJ.
     CAS# 6381-92-6 is not present on state lists from CA, PA, MN, MA, FL, or NJ.

California Prop 65

California No Significant Risk Level: None of the chemicals in this product are listed.

European/International Regulations

European Labeling in Accordance with EC Directives
Hazard Symbols:
Risk Phrases:

     R 36/37/38 Irritating to eyes, respiratory system and skin.
     R 60 May impair fertility.
     R 61 May cause harm to the unborn child.

Safety Phrases:

     S 26 In case of contact with eyes, rinse immediately with plenty of
     water and seek medical advice.
     S 45 In case of accident or if you feel unwell, seek medical advice
     immediately (show the label where possible).
     S 53 Avoid exposure - obtain special instructions before use.

WGK (Water Danger/Protection)

     CAS# 77-86-1: 1
     CAS# 10043-35-3: 1
     CAS# 6381-92-6: 2
Canada - DSL/NDSL
     CAS# 77-86-1 is listed on Canada's DSL List.
     CAS# 10043-35-3 is listed on Canada's DSL List.
     CAS# 6381-92-6 is listed on Canada's DSL List.
Canada - WHMIS
     This product has a WHMIS classification of D2B, D2A.
This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations and the MSDS contains all of the information required by those regulations.
Canadian Ingredient Disclosure List
     CAS# 10043-35-3 is listed on the Canadian Ingredient Disclosure List.

Section 16 - Additional Information

MSDS Creation Date: 8/14/1998
Revision #5 Date: 6/29/2007

The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. In no event shall Fisher be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages, howsoever arising, even if Fisher has been advised of the possibility of such damages.