Laboratory Assistant

BioPharma II: Molecular Biology Techniques

This course is a combination of lectures and hands-on laboratories in molecular biology and recombinant DNA techniques. Students learn hands-on laboratory techniques found in most molecular biology laboratories. Topics include DNA and RNA structure, media preparation, restriction enzymes, growth of bacteria, replication, transcription, and translation. The course also covers the use of plasmids and vectors, agarose gel electrophoresis, PCR, Southern and Western blotting, isolation of DNA, and digestion and analysis of DNA. Prerequisite: BioPharma I: Bioscience Lab Techniques or college-level bioscience courses. 148 hours. $295

52641 TTh 6/2–10/6 6–10 p.m. NDC 110 May 26 registration deadline

BioPharma III: Animal Cell Culture Techniques

This course provides students with a sound practical and theoretical knowledge of key techniques required to successfully initiate and maintain animal cell culture. Topics include aseptic techniques, growth environment, routine maintenance of cell cultures, cryopreservation, transfection, and cell counting. Other topics include cloning techniques, suspension culture, serum-free culture, evaluation of cryopreserved cells, extraction of DNA from mammalian cells, and use of multi-well plates in cell culture. Upon completion, students demonstrate the knowledge and skills required to grow, maintain, and manipulate cells in culture. Prerequisite: BioPharma I: BioScience Lab Techniques or college-level biology and chemistry courses. 152 hours. $295

52483 MW 7/25–12/5 6–10 p.m. NDC 110 July 18 registration deadline